Coding
Part:BBa_K4367011:Design
Designed by: Anders Kllberg Group: iGEM22_Chalmers-Gothenburg (2022-10-11)
FRET Protein (FP)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1504
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 14
Illegal BsaI.rc site found at 1511
Design Notes
This is elaborated in the long description. This part has been designed in accordance with the golden gate assembley method called MoClo. As such it contains associated MoClo overhangs. The 6xHis-tag is included for protein extraction.
Source
mRuby3 is derived from Entacmaea quadricolor, and mNeongreen is derived from Branchiostoma lanceolatum.